產(chǎn)品編號 | bs-0287R |
英文名稱 | Rabbit Anti-His tag antibody |
中文名稱 | His tag標簽抗體 |
別 名 | HisX6; 6His; His-Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; Histidine. |
Specific References (17) | bs-0287R has been referenced in 17 publications.
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產(chǎn)品類型 | 標簽抗體 |
研究領(lǐng)域 | 腫瘤 細胞生物 免疫學 染色質(zhì)和核信號 信號轉(zhuǎn)導 合成與降解 結(jié)合蛋白 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | Recombinant protein |
產(chǎn)品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | Synthetic peptide: HHHHHH conjugated to KLH |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
SWISS: N/A Gene ID: N/A 標簽抗體 蛋白質(zhì)純化的新途徑─應用His-Tag抗體檢測His-Tag融合蛋白 1) 6xHis與不同的Sepharose純化填料結(jié)合(捆綁),可不受構(gòu)象的影響,在天然或變形的條件下可進行進一步純化。 |
產(chǎn)品圖片 | |
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關(guān)于肽鏈的設(shè)計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |